This kit can be used to investigate the evolution of a bacterial population's resistance to antibiotics over generations. Simulate the process of natural selection using a robust protocol designed for the classroom. High school laboratory investigation with enough materials for 8 lab groups.
Time Requirement: 270 minutes over 2 weeks. Setting up the experiment, 1 lab period. Collecting data over the next 5 days, 15 to 20 minutes a lab period. Discussion and assessment, 2 to 3 lab periods.
- What’s Included:
3 Glass Beads, Pack
160 Culture Tubes
40 Sterile Petri Dishes
2 Sterile LB Agar, Bottle, 400 mL
8 Sterile LB Broth, 50 mL
20 Disposable Inoculating Loops
1 Nichrome Wire Inoculating Loop
1 Teacher’s Manual with Reproducible Student Guide
Not Included (these must be ordered from Carolina):
1 MM294 Slant Culture
1 MM294/pAMP Slant Culture
1 Ampicillin Vial, 10 mg/mL, 4 mL
8 Ampicillin Vial, 100 mg/mL, 200 µL
ORDER SEPARATELY:
Incubator, 37° C
Micropipets for 10-, 20-, 22-, and 100-µL Volumes
Micropipet Tips
Permanent Markers
Culture Tube Racks
Hot Pad or Gloves
Microcentrifuge Tubes, Sterile